restriction fragment的例句大全

Restriction endonuclease and target fragment PCR detection results show that the expression vector was constructed successfully.

经过酶切鉴定和目的片段PCR检测鉴定，证明表达载体构建成功。

The target fragment was digested by restriction enzyme to determine the accuracy of the detection technology.

用限制性内切酶酶切的方法判断该检测技术的准确性。

One of two positive clones was sequenced and analyzed by Peculiar primers directIy. The results of sequencing and restriction analysis indicated that the positive clone contains the part of human alpha-lactalbumin gene. The physical mny of about 10 kb fragment of the positive clone was made.

特异引物直接对粘粒阳性克隆进行部分序列测定及鉴定分析，结果进一步证明该阳性克隆含有不完全的人α-乳清白蛋白基因，并绘出了该阳性克隆约10kb的片段物理图谱。

Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

It enables a specific gene to be located on a particular restriction enzyme fragment.

它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。